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Unravelling Pasteurella multocida Toxin (PMT): Structural insights, antibiotic assessment, and identification of

Subodh Soni1, Priyanka2, Pooja Chugh1

  • 1Centre for Medical Biotechnology, Maharshi Dayanand University, Rohtak, Haryana 124001, India.

Computational Biology and Chemistry
|June 28, 2025
PubMed

Insights

New antibiotics like Amikacin, Ertapenem, and Tigecycline show high efficacy against Pasteurella multocida toxin (PMT). This study identifies promising therapeutic agents to combat severe bacterial infections in veterinary and human medicine.

Area of Science:

  • Microbiology
  • Structural Biology
  • Computational Chemistry

Background:

  • Pasteurella multocida is a significant bacterial pathogen causing severe infections in various hosts.
  • The Pasteurella multocida toxin (PMT) is a key virulence factor responsible for tissue damage and diseases like atrophic rhinitis.

Purpose of the Study:

  • To investigate the structural basis of PMT.
  • To identify potential antibiotic inhibitors against PMT using computational and experimental methods.

Main Methods:

  • Experimental determination of wild and mutant-type PMT proteins.
  • Computational virtual screening and molecular docking.
  • Molecular dynamic simulations and MMPBSA analysis.
  • Antibiotic Sensitivity Testing (AST) and disc diffusion tests.

Main Results:

  • Amikacin, Ertapenem, and Tigecycline demonstrated superior efficacy against PMT.
  • Disc diffusion tests validated the sensitivity and effectiveness of these antibiotics.
  • The identified antibiotics showed greater efficacy compared to the standard drug.

Conclusions:

  • Amikacin, Ertapenem, and Tigecycline are promising therapeutic agents against PMT.
  • This research provides potential inhibitors for Pasteurella multocida infections.
  • Findings address critical needs in veterinary and microbiological treatment strategies.
Keywords:
ASTMolecular Dynamic SimulationsMolecular dockingPMTPasteurella multocida

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