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Chitosan/PVA film containing β-acids/HP-β-CD inclusion complex for MRSA-infected wound healing

Hui Liu1, Shuanghe Li2, Shiyao Hua3

  • 1Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, 750004, China; Pharmacy Department, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, 750004, China; College of Pharmaceutical Sciences, Ningxia Medical University, Yinchuan, Ningxia, 750004, China.

International Journal of Biological Macromolecules
|June 28, 2025
PubMed

Insights

This study developed a novel bio-composite film using hops

Area of Science:

  • Biomaterials Science
  • Wound Healing
  • Antimicrobial Agents

Background:

  • Methicillin-resistant Staphylococcus aureus (MRSA) infections cause delayed wound healing, a major clinical issue.
  • Beta-acids from hops possess potent antibacterial and antioxidant properties but suffer from poor water solubility.
  • Limited solubility of beta-acids hinders their use in treating clinical wound infections.

Purpose of the Study:

  • To enhance the solubility of beta-acids using 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex (IC) formation.
  • To fabricate a chitosan/polyvinyl alcohol (CS/PVA) based bio-composite film incorporating the IC.
  • To evaluate the efficacy of the developed film as a wound dressing for MRSA-infected wounds.

Main Methods:

  • Inclusion complex (IC) formation between beta-acids and HP-β-CD.
  • Fabrication of a chitosan/polyvinyl alcohol (CS/PVA) bio-composite film containing the IC via solvent casting.
  • Characterization using FTIR, XRD, SEM, TGA; assessment of physical properties, antioxidant, release kinetics, antimicrobial activity, and in vivo wound healing in a mouse model.

Main Results:

  • Characterization confirmed successful integration and interaction of film components.
  • The bio-composite film demonstrated improved moisture content, water contact angle, water solubility, and water vapor permeability.
  • Significant in vitro antioxidant activity (>60%), accelerated beta-acid release at pH 7.4, potent MRSA antimicrobial activity, and accelerated wound healing (>99% healing area) in vivo.

Conclusions:

  • The developed 0.9HP-β-CD@CS/PVA film effectively enhances beta-acid solubility and exhibits strong antioxidant and antimicrobial properties.
  • The film demonstrates significant potential as an advanced wound dressing for treating MRSA-infected wounds.
  • This approach offers a promising strategy for utilizing natural compounds in combating antibiotic-resistant bacterial infections.
Keywords:
ChitosanCyclodextrinMRSAPolyvinyl alcoholWound healingβ-acids

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