SPDYE3 promotes cell cycle and LUSC progression by regulating the CDC25C/CDK1 pathway

Area of Science:

• Oncology
• Molecular Biology
• Cancer Research

Background:

• Lung cancer remains a leading cause of cancer mortality worldwide.
• Lung squamous cell carcinoma (LUSC) lacks targeted therapies.
• The role of the Speedy/Ringo gene family member SPDYE3 in LUSC is not well understood.

Purpose of the Study:

• To investigate the expression, clinical significance, and functional role of SPDYE3 in LUSC.
• To explore the underlying molecular mechanisms of SPDYE3 in LUSC progression.

Main Methods:

• Gene chip technology and qRT-PCR were used to analyze SPDYE3 expression in LUSC tissues, plasma, and saliva.
• In vitro and in vivo experiments assessed SPDYE3's impact on LUSC cell proliferation and cell cycle.
• Immunoprecipitation, mass spectrometry, and Western blotting identified interactions between SPDYE3 and CDC25C.

Main Results:

• SPDYE3 was found to be upregulated in LUSC tissues, plasma, and cells.
• SPDYE3 demonstrated diagnostic potential with an AUC of 0.7288.
• SPDYE3 promotes LUSC cell proliferation and cell cycle advancement by activating CDK1 via interaction with CDC25C.

Conclusions:

• SPDYE3 plays a novel regulatory role in LUSC cell cycle progression.
• The SPDYE3/CDC25C/CDK1 signaling pathway is implicated in LUSC pathogenesis.
• SPDYE3 represents a potential diagnostic biomarker and therapeutic target for LUSC.