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Updated: Sep 17, 2025

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells
Published on: March 3, 2015
NGPINT V3: a containerized orchestration Python software for discovery of next-generation protein-protein
Schuyler D Smith1,2, Valeria Velásquez-Zapata1,2, Roger P Wise1,3
1Department of Plant Pathology, Entomology, and Microbiology, Iowa State University, Ames, IA 50011, United States.
Summary:
Batch yeast two-hybrid (Y2H) assays, leveraged with next-generation sequencing, have afforded successful innovations for the analysis of protein-protein interactions. NGPINT is a Conda-based software designed to process the millions of raw sequencing reads resulting from Y2H-next-generation interaction screens. Over time, increasing compatibility and dependency issues have prevented clean NGPINT installation and operation. A system-wide update was essential to continue effective use with its companion software, Y2H-SCORES. We present NGPINT V3, a containerized implementation built with both Singularity and Docker, allowing accessibility across virtually any operating system and computing environment.
Availability And Implementation:
This update includes streamlined dependencies and container images hosted on Sylabs (https://cloud.sylabs.io/library/schuyler/ngpint/ngpint) and Dockerhub (https://hub.docker.com/r/schuylerds/ngpint), facilitating easier adoption and integration into high-throughput and cloud-computing workflows. Full instructions and software can be also found in the GitHub repository https://github.com/Wiselab2/NGPINT_V3 and Zenodo https://doi.org/10.5281/zenodo.15256036.
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