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Updated: Sep 17, 2025
05:47
Isolation of Murine Lymph Node Stromal Cells
Published on: August 19, 2014
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Lymph node fibroblast phenotypes and immune crosstalk regulated by podoplanin activity
Spyridon Makris1, Yukti Hari-Gupta1, Jesús A Cantoral-Rebordinos2
1Stromal Immunology Group, MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK.
Cell Reports
|June 29, 2025
Summary
Podoplanin (PDPN) in adult lymph nodes regulates immune cell communication. Its absence increases inflammation and disrupts stromal-immune cell interactions, highlighting PDPN
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Lymph nodes are critical sites for immune responses.
- Fibroblastic reticular cells (FRCs) within lymph nodes mediate immune cell communication and function.
- Podoplanin (PDPN) is essential for lymph node development, but its role in adult FRCs is unclear.
Purpose of the Study:
- To investigate the role of PDPN signaling in adult FRCs.
- To determine the impact of PDPN deletion on lymph node immune function and stromal-immune cell crosstalk.
Main Methods:
- Conditional in vivo deletion of PDPN in FRCs using the PDGFRαmGFPΔPDPN model.
- Single-cell RNA sequencing (scRNA-seq) to analyze transcriptional changes.
- Assessment of immune signaling and stromal-immune cell interactions in steady-state and during adaptive immune responses.
Main Results:
- PDPN deletion in FRCs led to increased inflammatory signaling in lymph nodes.
- Disruption of stromal-immune cell crosstalk was observed in both steady-state and during immune responses.
- PDPN/CLEC-2 signaling was confirmed to alter FRC transcriptional states and immune-related gene expression.
Conclusions:
- PDPN expression in lymph node fibroblasts is crucial for regulating their immunoregulatory properties.
- PDPN acts as a key transcriptional regulator of fibroblast function in lymph nodes.
- Targeting PDPN signaling may offer therapeutic strategies for modulating lymph node immunity.
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