Assessing Urinary Microbiota in Chronic Cystitis Based on Midstream Urine Sample.
Margarita Boldyreva1, Maria Petrunicheva2, Anastasia Ivanova2
1NRC Institute of Immunology, Federal Microbiological Agency, Moscow, Russian Federation.
Urologia Internationalis
|June 29, 2025
Summary
Urinary tract infections (UTIs) and chronic cystitis show altered bacterial profiles. Key markers like genomic DNA and Lactobacillus spp. levels can help diagnose these urinary conditions.
Area of Science:
- Microbiology
- Urology
- Molecular Diagnostics
Background:
- Urinary tract infections (UTIs) are common, with chronic recurrent cystitis posing a significant health challenge.
- Understanding the urinary microbiota is crucial for diagnosing and managing urinary diseases.
Purpose of the Study:
- To compare the urinary microbiota in healthy women, women at risk for UTIs, and those with chronic recurrent cystitis.
- To identify potential molecular diagnostic markers for urinary tract conditions.
Main Methods:
- Multiplex real-time PCR was employed to analyze urine samples.
- Three patient groups were studied: healthy controls, chronic recurrent cystitis patients, and individuals at risk for UTIs.
Main Results:
- Chronic cystitis exhibited increased total bacterial mass (TBM) and genomic DNA, with a predominance of facultative anaerobes like Escherichia coli and Serratia marcescens.
- Increased genomic DNA and anaerobic microorganisms were found in UTI-risk patients.
- Both at-risk and chronic cystitis groups showed a reduction in Lactobacillus spp., more pronounced in chronic cystitis.
Conclusions:
- Genomic DNA, TBM, and Lactobacillus spp. levels serve as valuable molecular diagnostic markers.
- These markers can aid in diagnosing chronic cystitis, micronephrolithiasis, and bacterial vaginosis.
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