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RLIG1 promotes triple-negative breast cancer proliferation by activating mTORC1 signal pathway by interacting with

Qingqing Wang1, Fengxia Chen2, Bo Pei3

  • 1Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China; Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, China; Hubei Clinical Cancer Study Center, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, China.

Cellular Signalling
|June 29, 2025
PubMed
Abstract

Insights

RNA 5'-phosphate and 3'-OH ligase 1 (RLIG1) is elevated in triple-negative breast cancer (TNBC), promoting tumor growth. Inhibiting RLIG1 hinders cancer progression, suggesting it as a potential therapeutic target for TNBC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Triple-negative breast cancer (TNBC) has a poor prognosis, and its pathogenesis is not fully understood.
  • RNA 5'-phosphate and 3'-OH ligase 1 (RLIG1) expression is elevated in TNBC tissues and linked to reduced patient survival.

Purpose of the Study:

  • To investigate the role of RLIG1 in TNBC pathogenesis.
  • To explore RLIG1 as a potential therapeutic target for TNBC.

Main Methods:

  • Bioinformatics analysis, cell counting kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU) assays, colony formation, flow cytometry, and western blotting were used.
  • Mass spectrometry, co-immunoprecipitation, and immunofluorescence elucidated mechanistic insights.

Main Results:

  • RLIG1 inhibition impaired tumor cell proliferation and growth in vitro and in vivo.
  • RLIG1 acts as an oncogene, activating the mTORC1 signaling pathway and promoting ERK phosphorylation in TNBC cells.
  • RLIG1 interacts with ERK, driving cell cycle transition and proliferation.

Conclusions:

  • RLIG1 plays a significant role in TNBC progression.
  • RLIG1 represents a promising therapeutic target for triple-negative breast cancer.
Keywords:
ERKProliferationRLIG1TNBCmTORC1

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