Related Experiment Video
Updated: Jun 29, 2026

Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements
Published on: October 30, 2015
Pitfalls and other issues with the MTT assay
1Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
Abstract:
While the colorimetric assay commonly referred to as 'MTT' does provide an indication of effects of photodamage on mitochondrial enzyme levels, a careful analysis showed that the resulting data were not correlated with a clonogenic assay. While MTT data can be claimed to represent 'cytotoxicity', this is an undefined term and appears not to be necessarily correlated with the object of anti-tumor therapy: tumor eradication. Use of this assay for assessing effects of treatment on viability are therefore unreliable.
More Related Videos
Related Concept Videos
Multiple Comparison Tests
It would be easy to compare two samples using a significance alpha level of 0.05. In other words, there is only one sample pair to be compared. However, it would be difficult to identify a significantly different sample if the number...
Pulmonary Tuberculosis IV
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...

