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Inhibition of opioid growth factor receptor (OGFR) promotes wound healing in the freshwater pearl mussel (Hyriopsis schlegelii)

  • 0College of Life Science, Nanchang University, Nanchang, 330031, China.
Fish & Shellfish Immunology +

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June 29, 2025

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June 29, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

The opioid growth factor receptor (OGFR) in pearl mussels accelerates wound healing. Inhibiting HsOGFR speeds up tissue repair, suggesting its role in mollusk healing processes.

Area Of Science

  • Molluscan biology
  • Molecular biology
  • Regenerative medicine

Background

  • Hyriopsis schlegelii is a key freshwater pearl mussel species.
  • Pearl insertion causes significant damage to mussels.
  • Opioid growth factor receptor (OGFR) regulates cell proliferation and wound healing in other species.

Purpose Of The Study

  • To investigate the role of OGFR in Hyriopsis schlegelii wound healing.
  • To characterize the HsOGFR cDNA and its expression.
  • To determine the effect of HsOGFR modulation on tissue repair.

Main Methods

  • Rapid amplification of cDNA ends (RACE) to obtain HsOGFR cDNA.
  • Sequence analysis and domain identification.
  • RNA interference (RNAi) and pharmacological inhibition (naloxone) of HsOGFR.
  • Quantitative real-time PCR (qRT-PCR) for gene expression analysis.
  • Histological evaluation of wound healing.

Main Results

  • A 3662 bp cDNA for HsOGFR was identified, encoding 879 amino acids with conserved domains.
  • HsOGFR transcripts were found in all tested tissues, notably the hepatopancreas.
  • HsOGFR knockdown or inhibition significantly accelerated wound healing in mantle tissues.
  • HsOGFR mRNA levels decreased post-wounding, while cell cycle regulators (HsKi67, HsCCND, HsCDKL1, HsCDK6) increased after HsOGFR interference.

Conclusions

  • HsOGFR plays a crucial role in mollusk tissue repair.
  • Modulating HsOGFR impacts wound healing speed and cell cycle progression.
  • HsOGFR may mediate wound healing through interactions with cell cycle-related proteins.

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