Related Experiment Video

Updated: Sep 17, 2025

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis
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An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis

Published on: September 12, 2019

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Acteoside suppresses hepatocellular carcinoma progression via modulation of macrophage migration inhibitory factor

Liu-Lu Gao1, Dong Jia2, Jia-Qi Shi2

  • 1Department of Gastroenterology, Qin Huang Hospital, Xi'an 710600, Shaanxi, China.

International Journal of Biological Macromolecules
|June 29, 2025
PubMed

Insights

Acteoside (ACT) inhibits hepatocellular carcinoma (HCC) by targeting macrophage migration inhibitory factor (MIF) and MAPK signaling pathways. This natural compound suppresses tumor growth, migration, and invasion, offering a potential therapeutic strategy for HCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Hepatocellular carcinoma (HCC) progression is linked to elevated macrophage migration inhibitory factor (MIF) and mitogen-activated protein kinase (MAPK) signaling.
  • Understanding the molecular mechanisms underlying HCC is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the inhibitory effects of acteoside (ACT) on hepatocellular carcinoma (HCC) progression.
  • To elucidate the roles of MIF and MAPK signaling pathways in ACT's anti-cancer activity.

Main Methods:

  • Immunohistochemistry, in situ hybridization, CCK-8, wound healing, Transwell assays, Western blotting, PCR, and a rat HCC model were employed.
  • MIF knockdown and overexpression cell lines were utilized to assess MIF's function.
  • Analysis included cell proliferation, migration, invasion, autophagy, apoptosis, and metastasis-related protein expression.

Main Results:

  • MIF and MAPK signaling proteins were upregulated in HCC and cirrhotic tissues.
  • ACT significantly inhibited HCC cell proliferation, migration, and invasion in vitro.
  • ACT modulated key proteins involved in autophagy, apoptosis, and metastasis, both in vitro and in vivo.

Conclusions:

  • HCC progression is associated with increased MIF and MAPK activation.
  • Acteoside suppresses HCC by downregulating MIF and modulating MAPK phosphorylation.
  • ACT promotes autophagy and apoptosis while inhibiting invasion and migration, presenting a potential therapeutic avenue for HCC.
Keywords:
ActeosideHepatocellular carcinomaMAPK signaling pathway

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