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Updated: Aug 3, 2026
Neuronal Nuclei Isolation from Human Postmortem Brain Tissue
Published on: October 1, 2008
Application of Oral Mucosal Epithelial Cells in Noninvasive Pathological Diagnosis of Neuronal Intranuclear Inclusion
Xiaowen Li1,2, Yu Kong1,2, Ying Ji1,2
1Department of Neurology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Abstract:
We employed a novel method for collecting oral mucosal epithelial cells, an anagar-paraffin double-embedding technique for cell fixation in cytopathological processing. OMEC biopsy demonstrated a high diagnostic efficacy for NIID: H&E staining revealed inclusions in 60% (12/20) of patients, TEM confirmed them in 40% (8/20) of cases and immunofluorescence detected p62-positive aggregates in 9/20 (45%) and uN2CpolyG in 8/20 (40%) of cases, respectively. OMEC biopsy is a simple, noninvasive technique for detecting intranuclear inclusions in NIID, avoiding the need for invasive surgical procedures such as skin or labial gland biopsies.
