A novel splicing variant in TECTA associated with prelingual autosomal dominant nonsyndromic hearing loss via...

The TECTA gene encodes α-tectorin, the major non-collagenous glycoprotein of the tectorial membrane, and plays a critical role in intracochlear sound transmission. Unsurprisingly, mutations in TECTA underlie hearing loss in both mice and humans. Two forms of hearing loss are linked to TECTA mutations: DFNA8/12 (autosomal dominant) and DFNB21 (autosomal recessive). Using a combination of clinical examination, pedigree analysis, exome sequencing, and functional studies, we identified a novel aberrant splicing variant, c.5999G > A (p.Gly2000Glu), in TECTA as the cause of autosomal dominant hearing loss in five-generation kindred of Chinese descent and provided prenatal diagnosis for the family. To investigate whether the variant acts via a a dominant-negative effect, consistent with pathogenesis observed in mouse models, we performed in vivo RNA analysis. Our data demonstrated that the variant altered RNA splicing, specifically causing aberrant splicing of exon 20 and resulting in two in-frame deletions. Quantitative real-time polymerase chain reaction revealed no significant reduction in mRNA levels in lymphoblasts derived from individuals harboring the TECTA c.5999G > A (p.Gly2000Glu) variant or the TECTA c.5383 + 6 T > A splicing variant, previously shown to result in exon 16 skipping. This study confirms the involvement of an aberrant splicing mutation in TECTA in autosomal dominant nonsyndromic hearing loss, expands the mutational landscape of DFNA8/12 to include coding variants that alter RNA splicing, and underscores the importance of investigating splicing effects of coding variants.