Genome-wide identification of the GRAS gene family and evidence for the involvement of PgGRAS48 in main root development in Panax ginseng
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View abstract on PubMed
Summary
This summary is machine-generated.Researchers identified 139 GRAS genes in ginseng, discovering PgGRAS48 regulates main root growth by interacting with DELLA proteins via gibberellin pathways. This offers targets for improving medicinal plant root architecture.
Area Of Science
- Plant Molecular Biology
- Genomics
- Medicinal Plant Research
Background
- Panax ginseng (ginseng) is a vital traditional Chinese medicinal herb, with its roots prized for therapeutic properties.
- GRAS (GRI, RGA, SCR) genes are plant-specific transcription factors crucial for diverse physiological processes, including root development and hormone signaling.
Purpose Of The Study
- To systematically identify and characterize the GRAS gene family in the ginseng genome.
- To investigate the role of specific GRAS genes, particularly PgGRAS48, in regulating main root development.
- To elucidate the molecular mechanism underlying GRAS-mediated root elongation in ginseng.
Main Methods
- Genome-wide identification and bioinformatics analysis of 139 PgGRAS genes.
- Phylogenetic analysis, gene structure, and cis-acting element prediction.
- RNA-seq and qRT-PCR for expression analysis; functional validation via Arabidopsis overexpression, subcellular localization, molecular docking, BIFC, and Y2H assays.
Main Results
- 139 PgGRAS genes were identified, exhibiting diversity in protein structure and evolutionary patterns, including a novel PG28 subfamily.
- PgGRAS48, a SCL3 subfamily member, showed high root expression and was upregulated by GA, promoting main root elongation in Arabidopsis.
- PgGRAS48 interacts with PgGRAS2 (DELLA) in the nucleus, revealing a SCL3-DELLA regulatory mechanism in gibberellin-mediated root growth.
Conclusions
- The study provides a comprehensive analysis of the ginseng GRAS gene family, highlighting its role in root development.
- PgGRAS48 is identified as a key regulator of main root elongation, acting through the SCL3-DELLA-GA pathway.
- These findings offer valuable genetic resources and targets for improving root architecture in medicinal plants like ginseng.
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