Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

343
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
343
CRISPR01:59

CRISPR

53.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
53.0K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficient on-site detection of nitazenes in hair: Integrating MCX pipette-tip solid-phase extraction with miniaturized mass spectrometry.

Talanta·2026
Same author

Accelerating wastewater-based SARS-CoV-2 surveillance using a mobile laboratory in mixed infrastructure regions.

The Science of the total environment·2026
Same author

Molecular Solution to the Paradox of Ancient Brain Preservation.

Journal of proteome research·2026
Same author

Spatial Proximity in Single Atomic Co Sites Boosts Water-Derived Active Hydrogen for Efficient Neutral Nitrate-to-Ammonia Electrosynthesis.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

NSCLC brain metastases exhibit reduced HLA-I antigen presentation machinery and immune evasion independent of IFNγ signaling defects.

Molecular cancer·2026
Same author

Targeted Intracellular Delivery of Amino Acids to Trophoblast Cells Reveals Proteomic Signatures of Cellular Utilisation.

Biomolecules·2026

Related Experiment Video

Updated: Sep 17, 2025

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

22.3K

DNA-guided CRISPR/Cas12 for RNA targeting.

Piyush Jain1, Carlos Orosco1, Santosh Rananaware1

  • 1University of Florida.

Research Square
|June 30, 2025
PubMed
Summary

Researchers developed ΨDNA, a DNA-based guide for CRISPR-Cas12 enzymes, enabling precise RNA targeting and diagnostics. This innovation expands CRISPR applications beyond RNA guides for RNA detection and cellular modulation.

More Related Videos

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
08:37

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

7.7K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.4K

Related Experiment Videos

Last Updated: Sep 17, 2025

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

22.3K
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
08:37

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

7.7K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.4K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genetics

Background:

  • CRISPR-Cas systems are powerful tools for genome editing, RNA editing, and diagnostics.
  • Current CRISPR applications primarily rely on RNA-guided systems, limiting their scope.
  • Developing DNA-based guides offers a novel approach to expand CRISPR functionalities.

Purpose of the Study:

  • To introduce ΨDNA, a DNA-based guide for Cas12 enzymes, for efficient and specific RNA targeting.
  • To evaluate ΨDNA's efficacy in RNA detection and cellular RNA modulation.
  • To establish ΨDNA as a viable alternative to RNA guides in CRISPR-Cas12 applications.

Main Methods:

  • Engineering ΨDNA, a DNA-based guide mimicking crRNA in reverse orientation, for Cas12 enzymes.
  • Assessing Cas12-RNA assembly and trans-cleavage activity without RNA components.
  • Testing ΨDNA for short and long RNA sensing, including Hepatitis C Virus (HCV) RNA detection in clinical samples.
  • Evaluating ΨDNA's ability to guide Cas12 enzymes for RNA targeting in cellular environments, including mRNA degradation and multiplex knockdown.

Main Results:

  • ΨDNA demonstrated stable Cas12-RNA assembly and activated trans-cleavage activity.
  • ΨDNAs effectively sensed both short and long RNAs, achieving 100% accuracy in detecting HCV RNA in clinical samples.
  • ΨDNAs successfully guided Cas12 enzymes for RNA targeting in cells, leading to enhanced mRNA degradation via ribosome stalling.
  • Multiplex knockdown of multiple RNA transcripts was achieved using ΨDNA-guided Cas12 enzymes.

Conclusions:

  • ΨDNA serves as a robust DNA-based alternative to traditional RNA guides for CRISPR-Cas12 systems.
  • This DNA-guided approach significantly enhances the potential of CRISPR-Cas12 for diagnostic applications.
  • ΨDNA enables targeted RNA modulation within cellular environments, opening new avenues for research and therapeutics.