Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effects of plyometric training on performance-related metrics in track and field athletes: a systematic review and meta-analysis of randomized controlled trials, with preliminary evidence on injury prevention.

BMC sports science, medicine & rehabilitation·2026

Same author

Dermatologic conditions associated with HIV among US adults across different racial and ethnic groups: A retrospective cohort study using TriNetX.

Journal of the American Academy of Dermatology·2026

Same authorSame journal

Evaluation of Type 1 Error Rates in Duplex Sequencing for Mutagenicity Testing Using Vehicle Control Data and Simulation Analyses.

Environmental and molecular mutagenesis·2026

Same author

Nonclinical Considerations for Support of Pregnancy and Lactation in Clinical Trials.

Birth defects research·2026

Same author

Prevalence and incidence of fibromyalgia and associated severity in the United States using MarketScan.

Pain reports·2026

Same author

Psilocybin Decreases Preference for Large Rewards Accompanied by Increased Activity of Parvalbumin Neurons With Perineuronal Nets in the Medial Prefrontal Cortex.

The European journal of neuroscience·2026

Same journal

Evaluating the Suitability of HepaRG Cells for Regulatory Micronucleus Testing: Comparing Results for a Diverse Set of 28 Chemicals to Regulatory Accepted TK6 Cells.

Environmental and molecular mutagenesis·2026

Same journal

Resveratrol Mitigates Haloacetaldehyde Disinfection By-Products (HAL-DBPs)-Induced Oocyte Developmental Toxicity via Mitochondria-Dependent Pathways.

Environmental and molecular mutagenesis·2026

Same journal

DZNep: A Methyltransferase Modulator for Disease Mitigation.

Environmental and molecular mutagenesis·2026

Same journal

Transcriptional Dysregulation of Histone Methyltransferase Genes and Chromosomal Instability Associated With Chronic Pesticide Exposure.

Environmental and molecular mutagenesis·2026

Same journal

Benzo[b]fluoranthene Induces Mutation Accumulation and Cancer-Relevant Mutational Signatures in Mouse Lung Alongside Steady State Levels of Chromosome Damage in Blood.

Environmental and molecular mutagenesis·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 17, 2025

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

Transferability, Reproducibility and Sensitivity of Mutation Quantification by Duplex Sequencing.

Shaofei Zhang¹, Barbara L Parsons², Devon Fitzgerald³

¹Pfizer Worldwide Research, Development, and Medical, Groton, Connecticut, USA.

Environmental and Molecular Mutagenesis

|June 30, 2025

Summary

This summary is machine-generated.

Duplex Sequencing (DS), an error-corrected next-generation sequencing method, demonstrates high reproducibility and sensitivity for mutagenicity testing across multiple laboratories. This technology is suitable for regulatory applications, accurately detecting mutations in genetic toxicology studies.

Keywords:

ecNGS mutation frequency mutational spectra power analysis reconstruction experiment

More Related Videos

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

Related Experiment Videos

Last Updated: Sep 17, 2025

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

Area of Science:

Genetics
Toxicology
Biotechnology

Background:

Duplex Sequencing (DS) is an ultra-accurate, error-corrected next-generation sequencing (ecNGS) technology.
Regulatory mutagenicity testing requires validated, reproducible methods.

Purpose of the Study:

To evaluate the transferability, reproducibility, and sensitivity of DS for regulatory mutagenicity testing.
To assess the suitability of DS across different laboratories, including those with and without prior experience.

Main Methods:

A reconstruction experiment was designed using DNA from control and mutagen-treated rats (benzo[a]pyrene and N-ethyl-N-nitrosourea).
Mutation frequency (MF) standards were created by mixing DNA samples.
These standards were distributed to eight laboratories for DS library preparation and analysis.

Main Results:

All participating laboratories successfully prepared DS libraries and generated high-quality sequencing data.
Measured mutation frequencies and spectra were highly consistent across all sites.
DS reliably detected a 2-fold increase in mutation frequency compared to controls.

Conclusions:

Duplex Sequencing demonstrates high technical reproducibility and transferability for mutagenicity assessment.
The sensitivity and consistency of DS support its suitability for regulatory genetic toxicology testing.