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Related Experiment Video

Updated: Sep 17, 2025

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

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Zwitterionic Thiophene-Functionalized Sensing Interface Enables Robust Antifouling Electrochemical Analysis.

Jiamei Chen¹, Xinwei Hu¹, Yang Huang¹

¹Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, the NMPA and State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou 511436, China.

Analytical Chemistry

|June 30, 2025

Summary

This study presents a novel conductive and antifouling electrochemical sensor for rapid antibody detection in complex samples. The developed sensor demonstrates high sensitivity and potential for clinical applications without sample purification.

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Area of Science:

Electrochemistry
Materials Science
Biomedical Engineering

Background:

Electrochemical sensing interfaces require efficient electron transfer and resistance to nonspecific adsorption for accurate analysis of complex samples.
Developing robust sensing platforms is crucial for reliable detection of biomarkers in biological fluids.

Purpose of the Study:

To synthesize a zwitterionic thiophene (Th-Zw) monomer for creating a conductive and antifouling electrochemical sensor.
To develop a one-step electrochemical detection strategy for antibodies in unprocessed samples.

Main Methods:

In situ electrodeposition of Th-Zw, graphene oxide, and antigen molecules onto screen-printed electrodes.
Fabrication of a zwitterionic thiophene-modified electrode for enhanced conductivity and antifouling properties.
Electrochemical detection of anti-p53 antibody and anti-COVID-19 IgG in spiked human plasma and blood samples.

Main Results:

The Th-Zw-modified electrode exhibited protein-resistant hydrophobicity, enhanced conductivity, and stable antigen immobilization.
Achieved low detection limits of 5.95 ng/mL for anti-p53 and 6.58 ng/mL for anti-COVID-19 IgG.
Demonstrated significant differences in anti-p53 detection between cancer patients and healthy volunteers, indicating clinical applicability.

Conclusions:

A novel conductive and antifouling electrochemical sensor was successfully developed.
The sensor enables rapid, sensitive, and label-free detection of antibodies in actual samples, including human plasma and blood.
This strategy offers a promising new approach for one-step electrochemical detection in clinical diagnostics.