Construction of Lymphoma Organoids and Applications in Tumor Microenvironment Research

Lymphoma is a class of malignant tumors originating from the lymphatic system, with a complex tumor microenvironment playing a critical role in tumor initiation, progression, and therapeutic response. In recent years, the rapid development of organoid technology has provided a novel platform for lymphoma research, enabling effective in vitro simulation of the lymphoma microenvironment. Although methods for establishing lymphoma organoids have gradually matured and related studies are increasing, challenges such as the lack of standardized protocols and incomplete microenvironment reconstruction remain. This review aims to summarize the techniques, characteristics, and applications of lymphoma organoids in tumor microenvironment research, exploring their potential in immunotherapy and disease mechanism studies. It seeks to offer new insights and directions for future lymphoma research.