A dual-mode detection strategy for alkaline phosphatase activity based on dimeric G-quadruplex.

Area of Science:

• Biochemistry
• Analytical Chemistry
• Clinical Diagnostics

Background:

• Alkaline phosphatase (ALP) is a key hydrolase in mammalian tissues, crucial for physiological processes.
• Elevated ALP levels are indicative of various diseases, including neurological disorders, diabetes, and liver cancer.
• Accurate ALP quantification is vital for clinical diagnosis and disease monitoring.

Purpose of the Study:

• To develop a novel dual-mode assay system for sensitive and quantitative detection of alkaline phosphatase (ALP).
• To utilize a G-quadruplex dimer (Di-G4) for signal enhancement in ALP detection.
• To provide a cost-effective and user-friendly method for clinical applications.

Main Methods:

• Development of a dual-mode assay (fluorescence and colorimetric) for ALP quantification.
• Incorporation of a G-quadruplex dimer (Di-G4) to amplify detection signals.
• Optimization of assay conditions to achieve wide linear ranges and low limits of detection.

Main Results:

• Achieved wide linear ranges: 1-200 U L⁻¹ (fluorescence) and 1-300 U L⁻¹ (colorimetric).
• Obtained excellent limits of detection: 0.79 U L⁻¹ (fluorescence) and 1 U L⁻¹ (colorimetric).
• Demonstrated successful application in detecting ALP in human serum samples with satisfactory results.

Conclusions:

• The novel dual-mode assay offers high sensitivity, simple operation, and low cost for ALP detection.
• This method shows significant potential for clinical diagnosis and disease management.
• The Di-G4 enhanced system provides a robust platform for quantitative enzyme assays.