3-Aminobenzamide-linked Multifunctional Nanoparticles: A Potent Strategy for Modulating PARP1 in Cervical Cancer...

Resistance to chemotherapy remains a major obstacle in effective cancer treatment. To address this challenge, we developed multifunctional Fe₃O₄@SiO₂(FITC)-BTN/FA/3AB nanoparticles aimed at selectively enhancing the therapeutic efficacy of 3-aminobenzamide (3AB) while minimizing systemic toxicity, particularly when combined with low-dose cisplatin chemotherapy. In this study, we demonstrate that these nanoparticles not only exhibit potent cytotoxic effects against lung adenocarcinoma cells but also show significant therapeutic potential in human cervical adenocarcinoma models. We systematically evaluated their performance through cellular uptake assay, viability assay, apoptosis analysis, single-cell colony formation assay, mitochondrial membrane potential (MMP) assay, and quantitative PCR (qPCR). Our findings reveal that the nanoparticle formulation efficiently facilitates the intracellular delivery of 3AB, leading to robust inhibition of tumor cell proliferation and migration. Overall, the Fe₃O₄@SiO₂(FITC)-BTN/FA/3AB nanoparticle system represents a promising platform for intratumoral therapy, offering a targeted strategy to potentiate the efficacy of PARP1 inhibition in DNA repair for cervical cancer treatment.