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Updated: Sep 17, 2025
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Author Spotlight: A Novel Method for Comprehensive Cell Component Analysis of Cerebral Blood Clots
Published on: July 21, 2023
702
Thrombus Composition in Cerebral Venous Thrombosis.
Ghil Schwarz1, Angelo Cascio Rizzo1, Martina Di Como2
1Department of Neurology and Stroke Unit, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.
Stroke Research and Treatment
|July 1, 2025
Summary
Cerebral venous thrombosis (CVT) thrombi are larger and contain more red blood cells (RBCs) than acute ischemic stroke (AIS) thrombi. This composition correlates with CT density, offering insights into CVT pathophysiology.
Area of Science:
- Neurology
- Pathophysiology
- Histology
Background:
- Histological analysis of thrombi aids in understanding pathophysiology.
- Cerebral venous thrombosis (CVT) requires further investigation into its unique thrombus characteristics.
Purpose of the Study:
- To analyze endovascular treatment (EVT)-retrieved CVT thrombi.
- To compare CVT thrombi with acute ischemic stroke (AIS) thrombi.
- To correlate CVT thrombus composition with computed tomography (CT) density.
Main Methods:
- Retrospective case-series of 5 CVT and 10 AIS cases treated with EVT.
- Histological staining (H&E, Picro Mallory, Prussian Blue) and immunohistochemistry (CD61, MPO, CD3, CD20, CD34, CD68, citH3).
- Quantification of thrombus components and calculation of relative CT density (rHU).
Main Results:
- CVT thrombi were significantly larger (185.6 mm² vs. 21.8 mm²) and had lower fibrin content (16.6% vs. 46.5%) compared to AIS thrombi.
- CVT thrombi were rich in red blood cells (RBCs) and showed signs of early organization.
- Low fibrin content and high RBC-to-fibrin ratio correlated significantly with increased CT density (rHU).
Conclusions:
- CVT thrombi are characterized by larger size, higher RBC content, and lower fibrin compared to AIS thrombi.
- Thrombus composition, particularly RBC and fibrin levels, correlates with CT density in CVT.
- These findings provide novel insights into CVT pathophysiology, warranting further validation.
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