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Related Concept Videos

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  1. Home
  3. Research Domains
  5. Biological Sciences
  7. Plant Biology
  9. Phycology (incl. Marine Grasses)
  11. Accelerating Moss Identification Through The Development Of Specific Dna Barcodes Based On The Whole Chloroplast Genome.
  1. Home
  3. Research Domains
  5. Biological Sciences
  7. Plant Biology
  9. Phycology (incl. Marine Grasses)
  11. Accelerating Moss Identification Through The Development Of Specific Dna Barcodes Based On The Whole Chloroplast Genome.

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Accelerating Moss Identification Through the Development of Specific DNA Barcodes Based on the Whole Chloroplast Genome.

Yanlei Liu1,2, Fengjiao Shen1,3,4, Lu Wang1

  • 1College of Life Sciences, Hebei Normal University, Shijiazhuang, China.

Molecular Ecology Resources
|July 1, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

Identifying reliable DNA barcodes is crucial for moss taxonomy. This study reveals specific chloroplast DNA regions, like atpB-rbcL, as effective for precise moss identification and phylogenetic analysis.

Keywords:
DNA barcodesmolecular markersmossesphylogeny

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Area of Science:

  • Botany
  • Molecular Biology
  • Genomics

Background:

  • Mosses (bryophytes) are ecologically vital early land plants with horticultural and medicinal uses.
  • Accurate moss identification is challenging due to taxonomic complexity and a lack of specialists.
  • DNA barcoding offers a promising solution for precise moss identification.

Purpose of the Study:

  • To identify optimal DNA barcodes for mosses at the chloroplast genome level.
  • To develop reliable molecular markers for moss species identification and phylogenetic reconstruction.
  • To address limitations of existing markers not suited for mosses.

Main Methods:

  • Analysis of 61 complete moss chloroplast genomes from diverse taxa.
  • Selection of 12 highly variable regions as candidate DNA barcodes based on nucleotide polymorphism.
primer
species identification
  • Experimental validation of primer universality and DNA barcode efficacy using 103 samples.

    • Main Results:

    • Construction of the first family-level moss phylogeny using whole chloroplast genomes.
    • Identification of atpB-rbcL, psaI-accD, ycf2, ycf1, matK, rpoB-trnC, and clpP as effective DNA barcodes.
    • Demonstration of high primer universality (>90%) for newly designed markers.

    Conclusions:

    • The identified DNA barcodes enable accurate moss identification and phylogenetic analysis.
    • Inconsistencies in public chloroplast genome data highlight the need for standardized submissions.
    • Standardized data submission with reference genomes is recommended for future moss research.