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Updated: Sep 17, 2025

Determining Basal Energy Expenditure and the Capacity of Thermogenic Adipocytes to Expend Energy in Obese Mice
Published on: November 11, 2021
Female Energy Expenditure during Load Carriage in Thermal Environments
J Luke Pryor1, Riana R Pryor1, Adam W Potter2
1Center for Research and Education in Special Environments, Department of Exercise and Nutrition Sciences, University at Buffalo, Buffalo, NY.
Purpose:
To cross-validate the Load Carriage Decision Aid (LCDA) in healthy, military-aged, load carriage-naïve females in thermal environments.
Methods:
During seven experimental trials, varying from cool to hot environments (10°C, 20% relative humidity (RH); 22°C, 20% RH; 31°C, 20% RH; 40°C, 20% RH; 45°C, 20% RH; 31°C, 60% RH; 40°C, 60% RH), 27 females aged 18-35 yr walked at increasing speeds with and without load carriage. Paired mean differences between measured (indirect calorimetry) and estimated (LCDA) M˙ were evaluated using bias, concordance correlation coefficient (CCC), and statistical equivalency (±10%) tests.
Results:
LCDA estimates of M˙ were excellent in 22°C, 20% RH (Bias ± SD, -0.01 ± 0.33 W·kg -1 ; CCC, 0.992) with acceptable precision in other dry (20% RH) conditions: 10°C (-0.23 ± 0.39 W·kg -1 ; 0.969), 31°C (-0.13 ± 0.31W·kg -1 ; 0.989), 40°C (-0.24 ± 0.34 W·kg -1 ; 0.972), and 45°C (-0.25 ± 0.35 W·kg -1 ; 0.971). In humid conditions (60% RH), the LCDA performed well in 31°C (-0.15 ± 0.31W·kg -1 ; 0.983) but was unacceptable in 40°C (-0.33 ± 0.30 W·kg -1 ; 0.959). Statistical equivalence was detected for each environment except for 40°C, 60% RH (90% confidence interval, -10.1% to -6.7%).
Conclusions:
In females, the LCDA can confidently be used to estimate walking M˙ with and without load across a wide range of environments. Higher than expected energy costs should be expected in hot, humid conditions.
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