Bioinformatics analysis and expression of the transcription factor MYC2 in Brassica napus in response to salt stress

Salt stress is a significant factor limiting plant growth and can severely reduce crop yields. In addition to causing physiological damage, salt stress disrupts the plant's internal balance and hinders nutrient absorption. However, plants have developed various responses to combat this stress. This study focuses on one critical gene associated with the jasmonic acid pathway, known as BnMYC2, which plays an important role in enhancing salt resistance. The BnMYC2 gene activates downstream genes by binding to specific cis-acting element regions, thereby contributing to the plant's ability to withstand salt stress. We investigated the expression of the BnMYC2 gene in the roots, stems, and leaves of the Licord cultivar under salt stress. The experiment was designed completely randomly, with three replications. Our results indicate that BnMYC2 gene expression is higher in the roots compared to the stems and leaves. Notably, the expression level of the BnMYC2 gene peaked in the roots 12 h after the application of salt stress before subsequently decreasing. Bioinformatics analysis revealed that the BnMYC2 gene shares phylogenetic similarities with 16 genes in Arabidopsis thaliana and 8 genes in Glycine max. Additionally, the expression profile of the MYC2 gene in A. thaliana is 72.22% similar to that in the G. max genome. Overall, this research not only highlights the role of the MYC2 gene in salt tolerance but also provides valuable insights into the evolutionary aspects of the BnMYC2 gene, which can be beneficial for future studies related to genome analysis.