Modification of neutrophil chemotactic responsiveness during various inflammatory reactions

Various inflammatory reactions have been induced in order to examine the chemotactic response of polymorphonuclear leucocytes (PMN) collected under various experimental conditions. Cells were harvested from the pleural cavity of rats after the induction of three acute non specific inflammatory reactions and two immune reactions. The results obtained with two techniques of chemotactic assessment (agarose assay and Boyden chamber technique) demonstrated a variation of chemotactic response depending on the cell source and the chemoattractants used. Using agarose assay, we distinguished locomotor reactivity of PMN harvested after immune inflammatory reactions from that of PMN harvested after non immune inflammatory reactions. Chemokinetic and chemotactic responses to various chemoattractants were inhibited in the first case and not affected in the second. Using the Boyden chamber technique, inhibition of random or oriented migration of PMN harvested after immune inflammatory reactions after the injection of a non antigenic irritant such as calcium pyrophosphate crystals (CaPP) was also observed.