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Related Experiment Video

Updated: Sep 17, 2025

Fluorescence Lifetime Macro Imager for Biomedical Applications
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Chip-on-tip fluorescence lifetime imaging micro-camera toward endoscopic applications.

Dario Angelone, Noreen Nudds, Andrew B Matheson

    Optics Letters
    |July 1, 2025
    PubMed
    Summary
    This summary is machine-generated.

    We developed a 4mm fluorescence lifetime imaging micro-camera for endoscopy. This compact system achieves <30μm spatial resolution and <1ns temporal response, enabling detailed tissue imaging.

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    Area of Science:

    • Biomedical optics
    • Microscopy and imaging

    Background:

    • Fluorescence lifetime imaging (FLIM) provides functional information about biological tissues.
    • Miniaturization of FLIM systems is crucial for in vivo endoscopic applications.

    Purpose of the Study:

    • To develop and characterize a chip-on-tip micro-camera for endoscopic fluorescence lifetime imaging.
    • To evaluate the spatial and temporal performance of the micro-camera system.

    Main Methods:

    • Integration of a 128x120 single-photon avalanche diode (SPAD) array detector, imaging optics, and optical fiber into a 4mm diameter probe.
    • Characterization of spatial resolution using a test target (<30μm resolution achieved).
    • Measurement of temporal response across wavelengths (<1ns mean pixel impulse response width).

    Main Results:

    • Successful development of a compact, chip-on-tip FLIM micro-camera.
    • Demonstrated high spatial resolution (<30μm) and fast temporal response (<1ns).
    • Validation of performance through benchmarking with a time-correlated single-photon counting system.
    • Acquisition of fluorescence lifetime images from unlabeled ex vivo animal tissue samples.

    Conclusions:

    • The developed micro-camera is suitable for endoscopic applications requiring high-resolution functional imaging.
    • This technology advances in vivo biomedical imaging capabilities.
    • FLIM micro-camera enables label-free tissue characterization in endoscopic settings.