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Published on: March 30, 2019
Cascade DNA Logic Strategy for Profiling the MiRNA-210-Targeted Signaling Pathway in the Warburg Effect of
Jingyuan Yu1,2, Xiaojun Zhang1, Hao Sun1,2
1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.
Abstract:
The Warburg effect, a hallmark of cancer, entails a metabolic shift from oxidative phosphorylation to aerobic glycolysis and is tightly regulated by complex signaling pathways. Decoding the dynamic signaling pathways underlying oncogenic metabolic reprogramming remains a major analytical challenge. Here, we report a cascaded DNA logic gate strategy capable of profiling the miR-210/CYGB/p53 axis that regulates the Warburg effect in triple-negative breast cancer (TNBC) cells. By logically integrating an H2O2-responsive DNAzyme (Dz) module with an entropy-driven reaction (EDR), the INHIBIT-AND logic device simultaneously interrogates upstream (miR-210) and downstream (CYGB mRNA, H2O2) effectors, producing dual amplified fluorescent outputs in response to specific pathway states. This programmable system enables live-cell, logic-based decoding of the Warburg effect, distinguishing TNBC from other breast cancer subtypes and dynamically reflecting pathway modulation. Our approach establishes a generalizable framework for using molecular computation to analyze complex biological circuits, providing a powerful tool for precision pathway profiling and therapeutic strategy development.
