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Related Experiment Video

Updated: Sep 17, 2025

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

Published on: October 8, 2013

Label-Free Visualization of Ciliary Rootlets in Mouse Brain.

Yusuke Murakami^1,2, Mutsuo Nuriya^3,4, Zuliang Hu⁵

¹Ph.D. Program in Humanics, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan.

Analytical Chemistry

|July 1, 2025

Summary

This summary is machine-generated.

Researchers developed a new imaging method to visualize Rootletin, a key protein in neuronal primary cilia. This technique aids in understanding ciliary functions and neuronal development.

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Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Published on: September 5, 2018

Area of Science:

Neuroscience
Cell Biology
Biophysics

Background:

Neuronal primary cilia are crucial for brain development and function.
Rootletin is a structural protein essential for ciliary rootlets, but its role is poorly understood due to visualization challenges.

Purpose of the Study:

To develop a label-free, rapid, and sensitive imaging method for visualizing Rootletin in brain tissue.
To characterize the distribution and dynamics of ciliary rootlets in neurons.

Main Methods:

Utilized a multimodal multiphoton imaging platform combining second harmonic generation (SHG) microscopy with coherent anti-Stokes Raman scattering (CARS).
Implemented a one-step chemical pretreatment for background reduction in SHG imaging.
Applied the platform to mouse hippocampus tissue and cultured neurons.

Main Results:

Successfully visualized highly organized and specific intracellular distributions of neuronal ciliary rootlets.
Detected ciliary rootlets in both mature and immature cultured neurons.
Demonstrated the platform's sensitivity and applicability in neuroscience research.

Conclusions:

The developed label-free imaging platform offers a powerful tool for studying ciliary dynamics and neuronal function.
Provides new insights into the structural organization of neuronal ciliary rootlets.
Facilitates research in developmental neuroscience and neurodegenerative diseases.