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Constitutive ALS3 expression in Candida albicans has differential effects on yeast-locked tet-NRG1 and brg1Δ strains

Nicholas C Schena1, Kassandra M Baker1,2, Anna A Stark1,3

  • 1Department of Biomedical Sciences, Grand Valley State University, One Campus Drive, Allendale, MI, 49401, USA.

Folia Microbiologica
|July 2, 2025
PubMed

Insights

Candida albicans adhesin Als3p improves glass adhesion in yeast-locked strains. However, its effect on biofilm formation varies, decreasing in one mutant strain lacking hyphae.

Area of Science:

  • Microbiology
  • Mycology
  • Molecular Biology

Background:

  • Candida albicans utilizes surface proteins called adhesins for attachment.
  • Adhesins often exhibit specificity for different fungal morphological forms.
  • Hyphae-deficient strains typically show reduced surface adhesion and biofilm formation.

Purpose of the Study:

  • To investigate the role of the adhesin Als3p in yeast-locked Candida albicans strains.
  • To examine how ALS3 expression impacts adhesion and biofilm formation in strains with altered BRG1 and NRG1 gene expression.

Main Methods:

  • Expressed the ALS3 gene in Candida albicans strains with modified BRG1 and NRG1 gene expression.
  • Assessed the adhesion capabilities of these strains to glass surfaces.
  • Evaluated the biofilm-forming ability of the modified strains.

Main Results:

  • ALS3 expression enhanced the adhesion of both BRG1 and NRG1 mutant strains to glass.
  • Only one of the mutant strains (BRG1 or NRG1) exhibited altered biofilm formation.
  • The observed alteration in biofilm formation was a decrease.

Conclusions:

  • Als3p contributes to Candida albicans adhesion to surfaces, even in yeast-locked strains.
  • The regulatory genes BRG1 and NRG1 influence biofilm formation differently in response to ALS3 expression.
  • Understanding adhesin function in specific genetic backgrounds is crucial for controlling Candida albicans infections.
Keywords:
ALS3BRG1Candida albicansNRG1AdhesionBiofilms

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