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Related Experiment Video

Updated: Sep 17, 2025

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
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cTAGE5 is essential for adipogenesis and adipose tissue development.

Junwan Fan1, Tiantian Ma2, Xiaoping Ren3

  • 1China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. fanjunwan@ncrcnd.org.cn.

Nature Communications
|July 2, 2025
PubMed
Summary

Cutaneous T-cell lymphoma-associated antigen 5 (cTAGE5) is crucial for white adipose tissue development. Its absence in precursor cells impairs insulin signaling and cytoskeleton remodeling, leading to metabolic disorders.

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Area of Science:

  • Cell Biology
  • Metabolic Disorders
  • Adipogenesis

Background:

  • White adipocytes are key energy stores, but their specialization mechanisms during adipogenesis are unclear.
  • Dysfunctional adipocytes contribute to metabolic diseases.

Purpose of the Study:

  • To investigate the role of cutaneous T-cell lymphoma-associated antigen 5 (cTAGE5) in adipocyte differentiation and adipose tissue development.

Main Methods:

  • Conditional deletion of cTAGE5 in adipocyte precursor cells (APCs) in mice.
  • Analysis of insulin signaling, gene expression (actin cytoskeleton, lipid catabolism), and protein expression.
  • Assessment of adipocyte differentiation capacity using pharmacological inhibitors.

Main Results:

  • Conditional cTAGE5 deletion in APCs caused white adipose tissue loss and mortality.
  • cTAGE5 deficiency disrupted pro-insulin receptor processing and impaired insulin signaling.
  • Actin cytoskeleton remodeling defects and enhanced lipolysis were observed in adipocytes.

Conclusions:

  • cTAGE5 is essential for adipocyte differentiation and maturation.
  • cTAGE5 regulates adipogenesis via insulin signaling and cytoskeleton dynamics.
  • Targeting actin polymerization and lipolysis can rescue adipocyte differentiation defects.