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Updated: Sep 17, 2025

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
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Trioxane-based MS-cleavable cross-linking mass spectrometry for profiling multimeric interactions of cellular

Clinton Yu1, Eric Novitsky2, Sree Ganesh Balasubramani3

  • 1Department of Physiology & Biophysics, University of California, Irvine, Irvine, CA, USA.

Nature Communications
|July 2, 2025
PubMed
Summary

A new cross-linking method using tris-succinimidyl trioxane (TSTO) enables precise mapping of protein-protein interactions. This technique enhances structural biology by identifying complex protein networks and trimeric interactions with greater accuracy.

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