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Super-resolution Fluorescence Microscopy

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Related Experiment Video

Updated: Sep 17, 2025

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

Mapping the nuclear landscape with multiplexed super-resolution fluorescence microscopy.

Fariha Rahman¹, Victoria Augoustides², Emma Tyler¹

¹Lampe Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, North Carolina State University, Chapel Hill, NC, USA.

Nature Communications

|July 2, 2025

Summary

This summary is machine-generated.

This study visualizes nuclear organization using a novel imaging protocol. It reveals how active and repressive nuclear states organize from the microscale to the nanoscale.

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Last Updated: Sep 17, 2025

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

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Published on: June 24, 2017

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Published on: October 28, 2018

Area of Science:

Cell Biology
Molecular Biology
Genomics

Background:

The cell nucleus coordinates complex biological processes.
Understanding spatial organization of DNA, proteins, and epigenetic marks is crucial.
Existing imaging techniques have limitations in resolving nanoscale organization.

Purpose of the Study:

To develop a multiplexed imaging protocol for high-precision localization of nuclear targets.
To investigate the spatial organization of the nucleus across different length scales.
To reveal organizational principles of active and repressive nuclear states.

Main Methods:

Developed a multiplexed imaging protocol for nanometer-precision localization.
Analyzed spatial correlations of 13 nuclear targets within single cells.
Investigated the effects of perturbing histone acetylation and transcription on nuclear organization.

Main Results:

Nuclear specification into active/repressive states spans multiple length scales, strengthening at the nanoscale.
Heterochromatin and euchromatin exhibit unique nanoscale organizational principles.
Histone acetylation or transcription perturbation disrupted nanoscale organization but not microscale organization.

Conclusions:

The developed imaging and analysis pipeline provides insights into nuclear organizational principles.
Nanoscale organization plays a critical role in defining active and repressive nuclear states.
This approach can be applied to study other cellular compartments.