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Updated: Sep 17, 2025
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Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model
Published on: April 6, 2022
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Integrated transcriptomic and proteomic profiling of colonic tissue in interleukin-10-deficient mice
Lili Li1,2,3, Chunxiang Ma1, Kexin Chen3
1Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu, 610041, China.
Scientific Data
|July 2, 2025
Summary
Mice lacking interleukin-10 (IL-10) develop enterocolitis. Integrated transcriptomic and proteomic analysis revealed key molecular changes, identifying potential therapeutic targets for inflammatory bowel disease (IBD).
Area of Science:
- Immunology
- Gastroenterology
- Molecular Biology
Background:
- Interleukin-10 (IL-10) is a critical anti-inflammatory cytokine involved in inflammatory bowel disease (IBD) pathogenesis.
- Mice lacking IL-10 (IL-10-/-) spontaneously develop enterocolitis, serving as a model for IBD research.
Purpose of the Study:
- To investigate the molecular mechanisms underlying chronic enterocolitis in IL-10-/- mice.
- To identify novel signaling pathways and potential therapeutic targets for IBD by integrating transcriptomic and proteomic data.
Main Methods:
- Bulk RNA sequencing (RNA-seq) was utilized to analyze gene expression profiles of colonic tissue.
- Four-dimensional (4D) label-free mass spectrometry (MS) was employed for comprehensive proteomic analysis.
- Integrated omics data analysis was performed after rigorous quality control.
Main Results:
- A total of 635 differentially expressed genes and 1,071 differentially expressed proteins were identified.
- The integrated analysis highlighted specific molecular pathways altered in the colonic tissue of IL-10-/- mice.
Conclusions:
- This study provides a deep molecular understanding of colitis in IL-10 deficient mice.
- The identified differentially expressed genes and proteins represent potential therapeutic targets for treating inflammatory bowel disease (IBD).
