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LINC01518 functions as an oncogene in head and neck squamous cell carcinoma (HNSCC) by modulating miR-1-3p/Slug and

Swati1, Shraddha Tripathi1, Bakhya Shree1

  • 1Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, Hyderabad Campus, Jawahar Nagar, Kapra Mandal, Medchal District, Telangana, 500078, India.

Scientific Reports
|July 2, 2025
PubMed

Insights

Long non-coding RNA LINC01518 promotes head and neck squamous cell carcinoma (HNSCC) progression and metastasis. Targeting LINC01518 may offer a novel therapeutic strategy for HNSCC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Head and neck squamous cell carcinoma (HNSCC) is an aggressive malignancy with limited therapeutic options.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized as critical regulators in cancer development and progression.
  • The role of LINC01518 in HNSCC remains largely unexplored, despite its known function in other cancers.

Purpose of the Study:

  • To investigate the role and mechanism of LINC01518 in the pathophysiology of HNSCC.
  • To determine if LINC01518 could serve as a potential diagnostic or therapeutic target for HNSCC.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to assess LINC01518 expression in HNSCC tissues and cell lines.
  • In vitro loss-of-function experiments (siRNA-mediated knockdown) to evaluate the impact of LINC01518 on HNSCC cell behavior.
  • Analysis of cell proliferation, migration, invasion, and apoptosis assays.
  • RNA immunoprecipitation (RIP) and dual-luciferase reporter assays to elucidate the molecular mechanism involving microRNAs (miRNAs).

Main Results:

  • LINC01518 expression was significantly upregulated in high-grade HNSCC tumors compared to normal tissues.
  • Transforming growth factor-β (TGF-β) was identified as a promoter of LINC01518 expression in HNSCC cells.
  • LINC01518 knockdown suppressed HNSCC cell proliferation, migration, and invasion.
  • LINC01518 depletion enhanced cisplatin-induced apoptosis in HNSCC cells.
  • Mechanistically, LINC01518 functions as a competing endogenous RNA (ceRNA) by sponging miR-1-3p and miR-216b-5p, leading to the upregulation of their target genes, Slug and GRP78, respectively.

Conclusions:

  • LINC01518 plays a crucial oncogenic role in HNSCC by promoting cell proliferation, migration, invasion, and chemoresistance.
  • LINC01518 acts as a ceRNA for miR-1-3p and miR-216b-5p, influencing the expression of Slug and GRP78.
  • LINC01518 represents a promising therapeutic target for HNSCC.
Keywords:
miR-1-3pmiR-216b-5pHNSCCLINC01518TGF-βceRNA

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