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Mitochondria-related parameters of lymphocyte subsets can distinguish different disease stages in patients with HBV
Zhiqi Liu1, Bin Yin1, Liang Xu1
1The Second Hospital of Nanjing, Nanjing University of Chinese Medicine, Nanjing, China.
Insights
Mitochondrial parameters in T cells reveal distinct patterns across chronic hepatitis B, liver cirrhosis, and liver cancer stages. These findings introduce a novel biomarker for monitoring hepatitis B disease progression.
Area of Science:
- Immunology
- Hepatology
- Cell Biology
Background:
- Chronic hepatitis B (CHB) can progress to liver cirrhosis (LC) and hepatocellular carcinoma (HCC).
- Peripheral immune cell alterations, particularly T cell mitochondrial function, are implicated in disease progression but require detailed characterization.
Purpose of the Study:
- To investigate differences in peripheral immune indexes, focusing on T cell mitochondrial parameters, across CHB, LC, HCC, and healthy controls (HCs).
- To identify novel biomarkers for assessing hepatitis B disease progression.
Main Methods:
- Flow cytometry was used to analyze over 80 lymphocyte parameters, including mitochondrial mass and membrane potential, in 236 HBV-infected patients and 112 HCs (aged 40-59).
- Analysis focused on T cell subsets and their mitochondrial characteristics.
- A composite biomarker, MPOLS (mitochondrial parameters of lymphocyte subsets), was calculated.
Main Results:
- A sequential increase in memory T cell mitochondrial mass was observed from CHB to LC and HCC.
- A corresponding sequential decrease in mitochondrial low membrane potential ratio was noted across disease stages.
- Four key indicators were identified, and the MPOLS value effectively distinguished between HCs, CHB, LC, and HCC groups.
Conclusions:
- Mitochondrial parameters of peripheral lymphocytes serve as significant indicators for different stages of hepatitis B disease progression.
- The MPOLS offers a novel biomarker for early warning in high-risk hepatitis B patients.
- Combining MPOLS with clinical characteristics may enhance diagnostic accuracy and treatment strategies.
Abstract:
To explore the difference of peripheral immune indexes especially T subsets cell mitochondrial indexes in patients with chronic HBV infection (CHB), liver cirrhosis (LC), hepatocellular carcinoma (HCC) and healthy controls (HCs). From May 2023 to December 2023, HBV infected patients aged 40-59 years (HBV infection group, 236 cases) and healthy people of the same age group (control group, 112 cases) were selected as the study objects. More than 80 parameters related to lymphocyte including cell percentage, absolute count, mitochondrial mass and mitochondrial low membrane potential were obtained by flow cytometry. The mitochondrial mass of memory T cells was found to be sequentially raised in the disease progression of hepatitis B patients from CHB to LC and eventually to HCC, and mitochondrial low membrane potential ratio was sequentially decreased. Then we screened out 4 main indicators related to the stage of disease progression in HBV-infected patients and distinguished the differences among the control group, CHB, LC, and HCC groups through the calculation of MPOLS (mitochondrial parameters of lymphocyte subsets) value, which can be used as a novel biomarker for hepatitis B disease progression. Mitochondrial parameters of peripheral lymphocytes in HBV-infected patients are significant indicators of different stages of disease progression. The MPOLS provides rich clinical data support for clinical early warning of high-risk hepatitis B patients, and we believe that the MPOLS can be combined with a variety of clinical characteristics to achieve more accurate diagnosis and treatment.
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