Related Experiment Video
Updated: Aug 13, 2026

Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons
Published on: February 5, 2015
Exogenous uridine diphosphoglucuronic acid breakdown in rat small intestinal mucosa preparations. Effect of other
Abstract:
Breakdown of UDPGA incorporated in a mixture containing rat liver or small intestinal mucosa preparations was investigated in the absence of exogenous sugar acceptor. A rapid UDPGA breakdown was seen with intestinal mucosa preparations in such a way that UDPGA decreased to about 1% or less of the initial value after 15 min of incubation. It was estimated that 60 to 70% of total products derived from UDPGA at the end of the incubation with intestinal tissue was in the form of uridine (90%) and uracil (10%). The addition of UDPG, ATP or UDP-N-acetylglucosamine protected UDPGA against degradation by tissue. The results indicated that care must be taken during the incubation of exogenous UDPGA for glucuronyltransferase assay in the intestinal mucosa due to the predominance in this tissue of enzyme systems involved in UDPGA breakdown.
More Related Videos
08:46Regioselective O-Glycosylation of Nucleosides via the Temporary 2',3'-Diol Protection by a Boronic Ester for the Synthesis of Disaccharide Nucleosides
Published on: July 26, 2018
06:31Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids
Published on: June 2, 2023
Related Concept Videos
Drugs Affecting GI Tract Motility: Other Laxatives
Osmotic or saline laxatives, like magnesium hydroxide or milk of...
Methods for Studying Drug Absorption: In situ
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
Biosynthesis of Polysaccharides
Biosynthesis of Nucleic Acids