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Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
Published on: June 9, 2021
Study of changes in volatile flavor substances during the processing and storage of mutton ham
Xinyu Chen1, Huabo Fu1, Siqi Qiu1
1Key Laboratory of Agricultural Product Processing and Quality Control of Specialty (Co-construction by Ministry and Province), School of Food Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China.
Abstract:
This study systematically characterized the volatile profile of dry-cured mutton ham to elucidate the dynamic changes of its characteristic aroma compounds during processing and storage. Using complementary analytical approaches, gas chromatography-olfactometry-mass spectrometry (GC-O-MS) and headspace-gas chromatography-ion mobility spectrometry (GC-IMS) identified 69 and 70 volatile organic compounds (VOCs) during processing, respectively, with 63 and 82 VOCs detected during storage. Key odor-active compounds including 6-methylheptanol, benzaldehyde, 2-nonone, 2-hexanone, and 2-heptanone were identified as major contributors to the ham's aroma profile. Notably, the aroma characteristics evolved significantly during storage, developing intense sweet orange, earthy, and roasted notes. Multivariate analysis via partial least squares regression (PLSR) revealed significant correlations between VOCs and specific lipid species: PE (16:1/20:4), PE (24:0/18:1), PE (22:6/18:0), PC (18:3/14:1), PS (18:0/22:6), and SM (d18:1/26:1). The results demonstrate that lipid degradation initially dominated during processing, followed by progressive oxidation that became more pronounced during storage. This sequential transformation ultimately enhanced the development of characteristic volatile flavors in mutton ham.
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