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Exploring ENPP5 as a diagnostic biomarker for sepsis: a comprehensive bioinformatics analysis
Jiamin Gao1,2, Yanjun Li3,4, Jinping Huang3,4
1Laboratory of Infectious Disease, HIV/AIDS Clinical Treatment Center of Guangxi (Nanning), The Fourth People's Hospital of Nanning, Nanning, 530023, China. jiamingao000@163.com.
BMC Infectious Diseases
|July 2, 2025
Summary
Researchers identified ENPP5 as a key diagnostic biomarker for sepsis. This gene shows significant associations with immune cell infiltration, offering potential for new sepsis therapeutic strategies.
Area of Science:
- Genomics and Bioinformatics
- Immunology
- Molecular Biology
Background:
- Rising sepsis mortality necessitates novel therapeutic biomarkers.
- Current diagnostic methods lack sufficient reliability.
- Identifying functional biomarkers is crucial for improved sepsis management.
Purpose of the Study:
- To identify differentially expressed genes (DEGs) in sepsis.
- To construct a competing endogenous RNA (ceRNA) network.
- To discover novel diagnostic biomarkers for sepsis.
Main Methods:
- Differential expression analysis of mRNAs, lncRNAs, and miRNAs.
- Construction of a lncRNA-miRNA-mRNA ceRNA network.
- Application of LASSO and random forest models for biomarker identification.
Main Results:
- Identified 4,450 differentially expressed mRNAs (DEmRs) involved in the cell cycle.
- Seven hub genes were identified, with ENPP5 selected as the key diagnostic gene.
- ENPP5 correlated with immune cell infiltration, including neutrophils, macrophages, and T cells.
Conclusions:
- ENPP5 serves as a potential key biomarker for sepsis diagnosis.
- ENPP5's association with immune cells suggests therapeutic potential.
- This study provides a foundation for developing novel sepsis treatment strategies.
