Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Combinatorial Gene Control02:33

Combinatorial Gene Control

8.6K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Arthroscopic reconstruction of anterior cruciate ligament with preservation of the remnant bundle].

Zhongguo gu shang = China journal of orthopaedics and traumatology·2013
Same author

[Anterior cruciate ligament reconstruction with tendon graft enveloped by preserved remnants].

Zhongguo gu shang = China journal of orthopaedics and traumatology·2013
Same author

Genetic and molecular biological characterization of two homologous cheR genes from Leptospira interrogans.

Acta biochimica et biophysica Sinica·2013
Same author

Upregulation of glycoprotein nonmetastatic B by colony-stimulating factor-1 and epithelial cell adhesion molecule in hepatocellular carcinoma cells.

Oncology research·2013
Same author

Effect of implantation of biodegradable magnesium alloy on BMP-2 expression in bone of ovariectomized osteoporosis rats.

Materials science & engineering. C, Materials for biological applications·2013
Same author

[Texture variation of CC 5052 aluminum alloy slab from surface to center layer by XRD].

Guang pu xue yu guang pu fen xi = Guang pu·2013

Related Experiment Video

Updated: May 5, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.6K

Deciphering gene regulatory programs in mouse embryonic skin through single-cell multiomics analysis.

Qiuting Deng1,2,3, Pengfei Cai1,2,4, Yingjie Luo1,5

  • 1BGI Research, Shenzhen, 518083, China.

Genome Biology
|July 2, 2025
PubMed
Summary

We developed high-throughput single-cell chromatin accessibility and transcriptome sequencing (HT-scCAT-seq) to study embryonic mouse skin development. This method reveals gene regulatory mechanisms controlling skin cell differentiation and development.

Keywords:
Cis-regulatory elementsEmbryonic skin developmentHT-scCAT-seq

More Related Videos

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
08:30

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

13.4K
In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

6.6K

Related Experiment Videos

Last Updated: May 5, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.6K
Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
08:30

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

13.4K
In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

6.6K

Area of Science:

  • Developmental Biology
  • Genomics
  • Epigenetics

Background:

  • Cell type-specific transcriptional heterogeneity in embryonic mouse skin is known, but regulatory mechanisms remain understudied.
  • Existing methods lack the ability to simultaneously profile transcriptome and chromatin accessibility at single-cell resolution.
  • Investigating gene regulatory mechanisms is crucial for understanding embryonic skin development.

Purpose of the Study:

  • To introduce and validate high-throughput single-cell chromatin accessibility and transcriptome sequencing (HT-scCAT-seq) for dissecting gene regulatory mechanisms.
  • To elucidate the regulatory basis of epidermal stratification, periderm terminal differentiation, and fibroblast specification in embryonic mouse skin.
  • To identify key regulatory elements and transcription factors involved in embryonic skin development.

Main Methods:

  • Development and application of high-throughput single-cell chromatin accessibility and transcriptome sequencing (HT-scCAT-seq).
  • Simultaneous profiling of transcriptome and chromatin accessibility in single embryonic mouse skin cells.
  • In silico perturbation analysis and CUT&Tag experiments to validate transcription factor roles.

Main Results:

  • Identification of candidate cis-regulatory elements (cCREs) and their target genes critical for dermal and epidermal development.
  • Observation of distinct chromatin accessibility states in cells with similar gene expression profiles during periderm differentiation.
  • Characterization of ALX4 and RUNX2 as potential transcription factor regulators of dermal papilla lineage development.

Conclusions:

  • HT-scCAT-seq is a powerful tool for studying spatiotemporal gene regulation dynamics in single cells.
  • The findings enhance the understanding of embryonic skin development.
  • The study provides a scalable framework for investigating regulatory mechanisms in various biological systems and diseases.