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Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion
Published on: May 10, 2020
Bioinspired Immunomodulatory Scaffold Based on Mineralized Lotus Stalks Laden with MnCO Microspheres for Accelerated
Jianqiu Yang1,2, Lei Du2, Hui Du2
1Department of Orthopaedic Surgery, Institute of Orthopedics of Jiangxi Province and Jiangxi Provincial Key Laboratory of Spine and Spinal Cord Disease, the Second Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, 330006, China.
Abstract:
Bone regeneration remains a significant clinical challenge due to the complexity of the bone healing process and the need for biomaterials that provide both structural support and immunomodulatory functions. Here, a bioinspired immunomodulatory scaffold is developed, composed of mineralized decellularized lotus stalks (MDL) integrated with manganese carbonyl (MnCO)-loaded mesoporous polydopamine (mPDA) microspheres (MM@MDL3). This scaffold mimics the hierarchical architecture of natural bone while offering controlled CO and Mn2+ release, promoting M2 macrophage polarization, reducing inflammation, and enhancing osteogenesis. In vitro studies demonstrate that MM@MDL3 effectively promotes mesenchymal stem cell (MSC) differentiation by activating the BMP2/SMAD/RUNX2 pathway. In vivo rat calvarial defect models confirm significant bone regeneration, with increased bone volume, enhanced vascularization, and reduced osteoclastogenesis. These results demonstrate MM@MDL3 as a promising strategy for large-segment bone defect repair by integrating a biomimetic structure with immunomodulatory and osteogenic properties. The proposed scaffold has great potential for treating clinical large-segment bone defects.
