Related Experiment Video
Updated: Sep 17, 2025
10:10
Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
Published on: November 13, 2021
8.4K
Facile calixarene-based sensor array strategy for quality evaluation of Yinxing Mihuan oral solution
Kejing Niu1, Mengyu Ye1, Yun Lyu1
1State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine Tianjin 301617 China huijuanyu@tjutcm.edu.cn.
RSC Advances
|July 2, 2025
Summary
A new macrocycle-based fluorescence sensing strategy offers rapid quality control for traditional Chinese medicine (TCM) preparations like Yinxing Mihuan oral solution (YMOS). This method ensures batch consistency and precise raw material feeding, improving TCM manufacturing.
Area of Science:
- Analytical Chemistry
- Supramolecular Chemistry
- Traditional Chinese Medicine (TCM) Analysis
Background:
- Quality evaluation of Traditional Chinese Medicine (TCM) preparations requires efficient and rapid methods.
- Yinxing Mihuan oral solution (YMOS) is a clinical TCM preparation for cardiovascular and cerebrovascular diseases.
- Assessing batch-to-batch consistency and raw material feeding is crucial for TCM manufacturing.
Purpose of the Study:
- To develop an innovative macrocycle-based fluorescence sensing strategy for TCM quality evaluation.
- To design calixarene-based sensors for assessing YMOS batch consistency and raw material feeding.
- To demonstrate the feasibility of supramolecular fluorescence sensing for TCM.
Main Methods:
- Utilized indicator displacement assay (IDA) for molecular recognition between calixarenes and YMOS compounds.
- Converted molecular recognition into multi-fluorescence signals by extruding indicators.
- Employed theoretical calculations to elucidate recognition mechanisms.
- Constructed a three-element sensor array for quality consistency evaluation using a "spider-web" fluorescent fingerprint.
- Optimized the QAC5A·EY reporter pair for precise raw material feeding.
Main Results:
- Established a calixarene-based sensor array for evaluating YMOS quality consistency.
- Generated a standard fluorescent fingerprint for YMOS samples using a "spider-web" mode.
- Successfully applied the QAC5A·EY reporter pair to ensure precise raw material feeding during YMOS manufacturing.
- Demonstrated the effectiveness of the supramolecular fluorescence sensing method.
Conclusions:
- The calixarene-based sensor array provides a facile and high-throughput method for comprehensive YMOS evaluation.
- The developed strategy significantly improves the quality evaluation of TCM preparations.
- This approach facilitates efficient and rapid quality control in TCM manufacturing.
