Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ion-Exchange Chromatography

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Metal Hydroxide Composite with g-C<sub>3</sub>N<sub>4</sub> Catalyzed Oxidation of Antibiotics: A Step Towards Sustainable Development Goals for Antimicrobial Resistance.

Indian journal of microbiology·2026

Same author

Tailoring isomeric transformations and surface adsorption dynamics of glycopeptides under thermal and photolytic stress.

Analytical and bioanalytical chemistry·2025

Same author

Proteomic Profiling Unraveling the Role of Lactate Dehydrogenase a in Vascular Repair and Functional Recovery after Spinal Cord Injury.

ACS chemical neuroscience·2025

Same author

Malondialdehyde Mediated Alpha-Synuclein Aggregation: A Plausible Etiology of Parkinson's Disease in Oxidative Stress.

Chemical research in toxicology·2025

Same author

Strategic approaches to assess and quantify the oxidative stress biomarkers in complex biological systems.

Bioanalysis·2025

Same author

Glycated α-Synuclein Renders Glial Cell Activation and Induces Degeneration of Dopaminergic Neurons: A Potential Implication for the Development of Parkinson's Disease.

ACS chemical biology·2025

Search research articles

Related Experiment Video

Updated: Jun 25, 2026

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

Development of an assay method for tofersen using IPRP-LC-HRMS with an extracting ion chromatogram processing

Anuradha A Hargude¹, Vijay S Bhalekar¹, Ravi P Shah¹

¹Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research, Ahmedabad (NIPER-A), India. ravi.shah@niperahm.res.in.

Analytical Methods : Advancing Methods and Applications

|July 2, 2025

Summary

This study developed a new analytical method for tofersen (TSN) quantification, overcoming challenges with similar impurities. The validated assay ensures accurate measurement of tofersen in drug formulations.

More Related Videos

Author Spotlight: An Efficient Methodology to Confidently Differentiate and Characterize Fentanyl Analogs

Author Spotlight: An Efficient Methodology to Confidently Differentiate and Characterize Fentanyl Analogs

Published on: November 8, 2024

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

Related Experiment Videos

Last Updated: Jun 25, 2026

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

Author Spotlight: An Efficient Methodology to Confidently Differentiate and Characterize Fentanyl Analogs

Author Spotlight: An Efficient Methodology to Confidently Differentiate and Characterize Fentanyl Analogs

Published on: November 8, 2024

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

Area of Science:

Analytical Chemistry
Pharmaceutical Analysis

Background:

Oligonucleotide quality control is challenging due to co-eluting impurities, limiting assay applicability and regulatory compliance.
Accurate quantification of active pharmaceutical ingredients like tofersen (TSN) is hindered by structurally similar impurities.

Purpose of the Study:

To develop a robust analytical assay for accurate tofersen quantification.
To address the challenge of co-eluting impurities in oligonucleotide analysis.
To ensure compliance with regulatory standards for pharmaceutical quality control.

Main Methods:

Ion-pair reverse-phase liquid chromatography coupled with high-resolution mass spectrometry.
Utilized extracted ion chromatography processing for impurity resolution.
Employed Nusinersen (NSN) as an internal standard for signal stability.

Main Results:

The method demonstrated linear response and specificity within the 3-10 μg mL^-1 concentration range.
Recovery from the formulation matrix was within the acceptable 80-120% range.
The assay showed precision and robustness for critical chromatography and mass spectrometry parameters.

Conclusions:

The developed method enables accurate quantification of tofersen despite the presence of co-eluting impurities.
This validated assay meets ICH Q2(R2) and USP regulatory requirements.
The methodology provides a reliable tool for oligonucleotide quality control and pharmaceutical analysis.