Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

One-Pot Electrochemical Synthesis of (Hydroxyamino)methanesulfonate from CO<sub>2</sub>, Nitrate/Nitrite, and Sulfite.

Journal of the American Chemical Society·2026
Same author

Mechanisms of interaction between type 2 diabetes and psychological disorders and therapeutic interventions: a narrative review.

Frontiers in medicine·2026
Same author

Boron-Doped Carbon Coatings Stabilize Cu<sup>+</sup>/Cu<sup>0</sup> Interfaces to Promote Multicarbon Formation in CO<sub>2</sub> Electroreduction.

ChemSusChem·2026
Same author

Complementary constraints in germ and immune cells shape evolution of gene regulation and phenotype.

bioRxiv : the preprint server for biology·2026
Same author

3D chromatin compartment of round spermatids encodes the spatiotemporal program of histone-to-protamine exchange in spermiogenesis.

bioRxiv : the preprint server for biology·2026
Same author

Application of Isotopic Method to Identify the Sources of Nitrate in Freshwater: Principles, Biases, and Optimizations.

Water environment research : a research publication of the Water Environment Federation·2026

Related Experiment Video

Updated: Sep 17, 2025

Author Spotlight: Navigating Challenges and Innovations in Muscle Stem Cell Studies
07:09

Author Spotlight: Navigating Challenges and Innovations in Muscle Stem Cell Studies

Published on: March 1, 2024

886

Profiling Histone Modifications in Differentiating Mouse Spermatogonia with CUT&Tag.

Benjamin William Walters1, Haoming Yu1, Shubhangini Kataruka1

  • 1Department of Genetics, Yale School of Medicine, New Haven, CT, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 2, 2025
PubMed
Summary

Histone modifications in spermatogonia are crucial for fertility. A new protocol using Cleavage Under Targets and Tagmentation (CUT&Tag) enables detailed epigenetic analysis of these early male germ cells.

Keywords:
CUT&TagChromatinEpigeneticsEpigenomicsFlow cytometryHistone modificationSpermatogonia

More Related Videos

Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation
09:48

Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation

Published on: April 22, 2022

4.1K
Step-specific Sorting of Mouse Spermatids by Flow Cytometry
06:31

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Published on: December 31, 2015

10.8K

Related Experiment Videos

Last Updated: Sep 17, 2025

Author Spotlight: Navigating Challenges and Innovations in Muscle Stem Cell Studies
07:09

Author Spotlight: Navigating Challenges and Innovations in Muscle Stem Cell Studies

Published on: March 1, 2024

886
Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation
09:48

Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation

Published on: April 22, 2022

4.1K
Step-specific Sorting of Mouse Spermatids by Flow Cytometry
06:31

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Published on: December 31, 2015

10.8K

Area of Science:

  • Epigenetics
  • Spermatogenesis
  • Mammalian reproduction

Background:

  • Paternal histone modifications are vital for fertility and offspring development.
  • Current methods like ChIP-seq are limited for analyzing early spermatogenic cells (spermatogonia) due to low cell yields.
  • Understanding spermatogonial epigenetics is crucial for male reproductive health.

Purpose of the Study:

  • To develop a robust protocol for profiling histone modifications in spermatogonia.
  • To overcome the limitations of ChIP-seq for analyzing low cell numbers in early spermatogenesis.
  • To enable detailed investigation of the epigenome in premeiotic male germ cells.

Main Methods:

  • Combined fluorescence-activated cell sorting (FACS) with an optimized Cleavage Under Targets and Tagmentation (CUT&Tag) workflow.
  • Utilized as few as 50,000 dissociated, sorted cells.
  • Generated genome-wide histone modification enrichment data.

Main Results:

  • Achieved high-quality, reproducible CUT&Tag datasets from limited cell populations.
  • Successfully captured histone modification enrichment in differentiating spermatogonia.
  • Demonstrated the protocol's efficacy for analyzing the epigenetics of premeiotic germ cells.

Conclusions:

  • The developed FACS-optimized CUT&Tag protocol effectively profiles histone modifications in spermatogonia.
  • This method overcomes previous limitations, facilitating robust epigenetic studies in early male germ cells.
  • Enables easier and more reliable investigation of male germ line epigenetics.