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Related Experiment Video

Updated: Sep 17, 2025

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

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Published on: February 4, 2017

Spatial-offset pump-probe imaging of nonradiative dynamics at optical resolution.

Guo Chen¹, Yuhao Yuan¹, Hongli Ni¹

¹Department of Electrical and Computer Engineering, Boston University, Boston, MA 02215, USA.

Science Advances

|July 2, 2025

Summary

Spatial-offset pump-probe imaging (SOPPI) enables high-resolution mapping of photothermal and photoacoustic wave propagation. This new technique provides detailed insights into nonradiative field interactions within biological systems.

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Last Updated: Sep 17, 2025

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Published on: August 6, 2018

Area of Science:

Biomedical Optics
Acoustic Imaging
Photothermal Imaging

Background:

Nonradiative photothermal (PT) and photoacoustic (PA) processes are crucial for imaging, stimulation, and therapy.
High-resolution mapping of PA and PT wave generation and propagation is essential for understanding biological interactions.

Purpose of the Study:

To introduce spatial-offset pump-probe imaging (SOPPI) for simultaneous, high-resolution imaging of PA/PT wave propagation.
To demonstrate SOPPI's capability in visualizing complex wave phenomena and biological structures.

Main Methods:

SOPPI utilizes spatially offset pump and probe beams for simultaneous PA/PT wave imaging.
Achieved nanosecond temporal resolution, micrometer spatial resolution, and 65-MHz detection bandwidth.
Demonstrated sensitivity of 9.9-pascal noise equivalent pressure.

Main Results:

Successfully mapped PA and PT wave evolution and interaction with biological tissues (mouse skull and brain slices).
Recorded wavelength-dependent PA/PT generation, evanescent wave-generated PA, and acoustic Mach cone phenomena.
Developed SOPPI-based photoacoustic computed tomography (SOPPI-PACT) for precise pigment distribution reconstruction in zebrafish.

Conclusions:

SOPPI offers a powerful tool for high-resolution spatiotemporal mapping of PA and PT waves.
The technique provides unprecedented insights into nonradiative field dynamics in biological systems.
SOPPI-PACT enables precise reconstruction of biological structures with high signal-to-noise ratio.