The effect of antivenin on intramuscular pressure elevations induced by rattlesnake venom

The dose of an antivenin required to neutralize a clinical case of venom poisoning, as well as determining the timing or need to initiate antivenin treatment, is frequently difficult to objectively ascertain. In this study, venom from the southern Pacific rattlesnake, Crotalus viridis helleri, was injected into 29 dog hind limb anterolateral compartments. A solution of C. v. helleri venom (15 mg/ml) was prepared using dessicated venom and saline; 0.2 ml were injected sub-fascially so that each of the compartments received 3 mg of venom. In one group no antivenin was given, in a second group four vials of antivenin were administered i.v. 1 hr post-injection, and in the final group eight vials of antivenin were administered i.v. 1 hr following venom injection. In all groups intracompartment pressures, limb girth and surface temperature were measured at regular intervals over the first 48 hr. In the group receiving eight vials of antivenin the intracompartment pressure reached a peak mean pressure of 49 mm Hg at 2 - 4 hr, and then rapidly fell. In those treated with none or four vials the pressure rose to 70 and 60 mm Hg, respectively, and remained elevated over the first 24 hr. The difference between the former and latter two groups is statistically significant. The findings indicate that the intracompartmental pressure, and presumably destructive damage of the venom, can be controlled by adequate levels of i.v. antivenin. Intracompartmental pressure measurements should be considered as an adjunct in the monitoring and decision-making processes for the treatment of patients bitten by rattlesnakes.