Uncoupling of the calcium pump of the sarcoplasmic reticulum by kerosene

The effect of kerosene on the calcium pump of sarcoplasmic reticulum vesicles was investigated. Kerosene induces an increase in the rates of ATP hydrolysis without a change in calcium influx, so there is an overall decrease in the efficiency of the pump. These effects are dose-dependent. Our findings imply that kerosene induces an effect on the membrane of vesicles, reducing or inhibiting net calcium accumulation. It is concluded that kerosene induces an 'uncoupling' of the calcium pump through an alteration in the permeability of the membrane.