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Extraction: Advanced Methods

Extraction: Advanced Methods

Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...

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Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method

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Published on: April 11, 2020

Engineered phages for selective adsorption of rare earth elements.

Alexander M Ditzel¹, Scott N Dean², Ellen R Goldman²

¹Postdoctoral Fellowship for American Society for Engineering Education, Washington, DC, USA.

Scientific Reports

|July 2, 2025

Summary

This study developed phage display libraries for rare earth element (REE) extraction. A novel peptide-phage, H11G-p8, demonstrated preferential binding to terbium (Tb), showing potential for environmentally friendly REE separation.

Keywords:

Adsorption Engineered phages FRET Peptide Phage display Rare earth elements Tb

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Published on: December 6, 2015

Area of Science:

Biotechnology
Materials Science
Environmental Science

Background:

Biomaterial extraction of rare earth elements (REEs) presents an eco-friendly alternative to traditional chemical methods.
M13 phages can be engineered to display peptides for selective REE binding, minimizing non-specific interactions.

Purpose of the Study:

To develop diverse phage display libraries for identifying peptides with high affinity and selectivity for REEs.
To evaluate the potential of engineered phages for efficient and environmentally sound REE separation.

Main Methods:

Construction of two phage display libraries (12-mer and 17-mer peptides) with over 1x10^7 unique peptides each.
Preparation of fluorescent protein-peptide fusions for affinity (Kd) measurements.
Assessment of phage inhibition (Ki) values and direct REE binding (solution and immobilized).

Main Results:

Engineered phages exhibited REE affinities in the micromolar range.
A novel peptide-phage, H11G-p8, showed enhanced affinity for Europium (Eu).
Both H11G-p8 and E1 phages demonstrated preferential binding to Terbium (Tb), with H11G-p8 showing higher specific binding.

Conclusions:

H11G-p8 phage displays significant potential for selective Terbium (Tb) separation from other REEs.
Engineered phages offer a promising biomaterial-based approach for sustainable REE recovery.