Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An integrative model of FGF2-induced signaling and muscle cell proliferation.

Journal of theoretical biology·2026
Same author

Western Diet Downregulates Hepatic Bitter Taste Receptors: Potential Link to Altered Lipid Metabolism.

Molecular nutrition & food research·2026
Same author

Designing the MyoFusion Media: A Serum-Free Medium Optimized for Bovine Satellite Cell Differentiation.

Biotechnology and bioengineering·2026
Same author

Extra-oral expression of bitter taste receptors in pigs and their correlation with hepatic cytochrome P450 enzymes.

Cell and tissue research·2025
Same author

From Muscle Fibers to Functional Foods: Bridging Conventional and Cultivated Approaches.

Advances in biochemical engineering/biotechnology·2025
Same author

Impact of abiotic stress on miRNA profiles in tomato-derived extracellular vesicles and their biological activity.

International journal of biological macromolecules·2025

Related Experiment Video

Updated: Sep 17, 2025

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
09:19

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

Published on: February 15, 2021

5.0K

High-Throughput Label-Free Continuous Quantification of Muscle Stem Cell Proliferation and Myogenic Differentiation.

Stig Skrivergaard1, Martin Krøyer Rasmussen2, Margrethe Therkildsen2

  • 1Department of Food Science, Aarhus University, Aarhus, Denmark. stsk@food.au.dk.

Stem Cell Reviews and Reports
|July 2, 2025
PubMed
Summary

A new high-contrast brightfield imaging technique allows label-free, high-throughput assessment of muscle satellite cell proliferation and differentiation. This method provides detailed kinetic data for applications in muscle regeneration and cultivated meat research.

Keywords:
High-throughput screeningImage-based cytometryLabel-free imagingLive cell assaysMyogenesisMyotube quantificationSatellite cells

More Related Videos

Author Spotlight: Investigating Cellular and Molecular Dynamics During Muscle Regeneration Using Cutting-Edge Single-Cell Technologies
11:02

Author Spotlight: Investigating Cellular and Molecular Dynamics During Muscle Regeneration Using Cutting-Edge Single-Cell Technologies

Published on: December 1, 2023

1.2K
Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
11:22

Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle

Published on: January 12, 2015

16.2K

Related Experiment Videos

Last Updated: Sep 17, 2025

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
09:19

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

Published on: February 15, 2021

5.0K
Author Spotlight: Investigating Cellular and Molecular Dynamics During Muscle Regeneration Using Cutting-Edge Single-Cell Technologies
11:02

Author Spotlight: Investigating Cellular and Molecular Dynamics During Muscle Regeneration Using Cutting-Edge Single-Cell Technologies

Published on: December 1, 2023

1.2K
Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
11:22

Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle

Published on: January 12, 2015

16.2K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Muscle Physiology

Background:

  • Quantifying muscle satellite cell proliferation and differentiation is vital for muscle regeneration, disease modeling, and cultivated meat research.
  • Traditional fluorescence assays are sensitive but labor-intensive, endpoint-restricted, and disruptive to myotube integrity.

Purpose of the Study:

  • To develop a novel high-contrast brightfield (HCBF) imaging technique for high-throughput, label-free assessment of satellite cell proliferation and myogenic differentiation.
  • To enable continuous, time-resolved quantification without additional reagents or cell manipulation.

Main Methods:

  • Utilized the BioTek Cytation 5 automated imager and Gen5 software for HCBF imaging.
  • Optimized imaging parameters for 96- and 384-well formats.
  • Developed a label-free screening method for clonal myogenicity assessment in a 384-well format.

Main Results:

  • Enabled detailed kinetic profiling of satellite cell behavior, including myotube formation dynamics and species-specific media responses.
  • Demonstrated efficient myotube formation in serum-free media for bovine and porcine satellite cells, with distinct kinetics compared to serum-containing samples.
  • Highlighted well-to-well variation, myotube detachment, and a second wave of myotube formation in serum-containing samples.

Conclusions:

  • The HCBF methodology advances myogenic research by eliminating the need for genetic labeling, invasive staining, or specialized consumables.
  • Offers new opportunities for efficient screening and detailed kinetic data acquisition for serum-free media development, drug discovery, and pathophysiological testing.
  • Applicable to both cultivated meat and musculoskeletal research fields.