Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Riboswitches01:56

Riboswitches

8.7K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.7K
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

125
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
125
Translational Regulation01:29

Translational Regulation

108
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
108
Types of RNA01:23

Types of RNA

65.6K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
65.6K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.0K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.0K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling.

Nature communications·2026
Same author

Function Over Form: Defining Evolutionarily Conserved Long Noncoding RNAs Regulating Hepatic Metabolism.

Gastroenterology·2025
Same author

The endocytic adaptor AP-2 maintains Purkinje cell function by balancing cerebellar parallel and climbing fiber synapses.

Cell reports·2025
Same author

The molecular dissection of TRIM25's RNA-binding mechanism provides key insights into its antiviral activity.

Nature communications·2024
Same author

Free ribosomal proteins as culprits for nucleolar stress.

Molecular cell·2024
Same author

The RNA-binding protein landscapes differ between mammalian organs and cultured cells.

Nature communications·2023
Same journal

Publisher Correction: Chain splitting of insulin: an underlying mechanism of insulin resistance?

npj metabolic health and disease..·2025
Same journal

Incretin-based therapies for the treatment of obesity-related diseases.

npj metabolic health and disease..·2025
Same journal

Pre-conceptional paternal diet impacts on offspring testosterone homoeostasis via epigenetic modulation of cyp19a1/aromatase activity.

npj metabolic health and disease..·2025
Same journal

The role of NAD<sup>+</sup> metabolism and its modulation of mitochondria in aging and disease.

npj metabolic health and disease..·2025
Same journal

Chain splitting of insulin: an underlying mechanism of insulin resistance?

npj metabolic health and disease..·2025
Same journal

The effect of high-sugar feeding on rodent metabolic phenotype: a systematic review and meta-analysis.

npj metabolic health and disease..·2025
See all related articles

Related Experiment Video

Updated: Sep 17, 2025

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

23.9K

RNA-binding proteins as versatile metabolic regulators.

Ellie Koletsou1,2, Ina Huppertz3,4

  • 1Max Planck Institute for Biology of Ageing, Joseph-Stelzmann-Straße 9b, 50931, Cologne, Germany.

Npj Metabolic Health and Disease
|July 2, 2025
PubMed
Summary
This summary is machine-generated.

RNA-binding proteins (RBPs) are crucial regulators of cellular metabolism across various biological processes. This review details how RBPs control energy pathways, impacting development, homeostasis, and disease.

More Related Videos

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

6.8K
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using &#967;CRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

5.2K

Related Experiment Videos

Last Updated: Sep 17, 2025

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

23.9K
Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

6.8K
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using &#967;CRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

5.2K

Area of Science:

  • Cellular Metabolism
  • Molecular Biology
  • Biochemistry

Background:

  • Metabolic shifts are fundamental to cellular processes like stem cell differentiation and immune cell activation.
  • These metabolic changes are critical throughout embryonic development, adult tissue homeostasis, and various disease states.

Purpose of the Study:

  • To review the regulatory role of RNA-binding proteins (RBPs) in coordinating cellular metabolic shifts.
  • To explore how RBPs modulate key energy pathways, including glycolysis and oxidative phosphorylation.
  • To examine the mechanisms by which RBPs influence metabolic gene expression and function.

Main Methods:

  • Literature review of studies on RNA-binding proteins and cellular metabolism.
  • Analysis of RBP mechanisms in regulating metabolic pathways.
  • Examination of RBP function in development, homeostasis, aging, and disease.

Main Results:

  • RBPs actively regulate post-transcriptional control of metabolically relevant genes.
  • RBP function can be modulated by RNAs, metabolites, and growth factors, impacting cellular energy demands.
  • Ageing and disease can alter RBP function, potentially disrupting metabolic regulation.

Conclusions:

  • RBPs play a critical role in orchestrating cellular metabolism.
  • Understanding the interplay between RBPs and metabolism offers therapeutic potential for regenerative medicine and age-related diseases.