Related Experiment Video For causal association
Updated: Sep 17, 2025

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
Published on: June 5, 2020
The Potential Carcinogenicity of Long-Term Polymetallic Exposure on the Development of Primary Liver Cancer: Nested
Lizhen Zhang1,2, Yana Bai2, Yan Cheng3
1Department of Medical Records, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu, P. R. China.
Abstract:
With the rapid development of global metal exposure, while there is evidence indicating the toxicity of individual metals, the combined impact of mixed exposure of multiple metals on primary liver cancer (PLC) risks remains inadequately characterized. Therefore, we conducted this nested case-control study within the Jinchang cohort, including 129 incidents of PLC cases after 10 years of follow-up and 387 healthy controls matched by propensity score matching methods. Our findings showed that long-term exposure of chromium (Cr, Q2: OR = 10.63, 95% CI: 4.21-26.81; Q3: OR = 5.42, 95% CI: 2.14-13.70), zinc (Zn, Q2: OR = 2.37, 95% CI: 1.10-5.11; Q3: OR = 2.79, 95% CI: 1.32-5.88; Q4: OR = 2.83, 95% CI: 1.33-5.98), iron (Fe, Q4: OR = 0.26, 95% CI: 0.12-0.60), and aluminum (Al, Q4: OR = 0.26, 95% CI: 0.11-0.64) was identified as key contributing factors of the incidents of PLC. Serum Cr exhibited a nonlinear relationship with PLC risk (ρoverall < 0.001, ρnonlinear < 0.001), whereas Zn demonstrated a near-linear positive trend (ρoverall = 0.023, ρnonlinear = 0.395). In contrast, Fe, lithium (Li), and Al showed inverse associations with PLC incidence. Sensitivity analyses further confirmed these relationships. Additionally, significant interactions were observed among serum Cr, Zn, Fe, and Al in modulating PLC risk (ρinteraction < 0.05). These results highlighted the complex dose-dependent effects and interactions of multimetal exposures in the PLC pathogenesis and underscored the need for further mechanistic investigations.
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