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Updated: Aug 12, 2026

A Method for Extracting Pigments from Squid Doryteuthis pealeii
Published on: November 9, 2016
Isolation, identification, and production optimization of natural functional pigments produced by Talaromyces
Xian Xia1,2, Li-Yu Liu1, Miao Liu1
1Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, China.
Abstract:
Natural microbial pigments are gaining attention for their potential in various applications due to their safety and sustainability. In this study, we isolated a high-yielding pigment-producing fungus identified as Talaromyces atroroseus LWT-1. The predominant pigment compounds were isolated from the culture extract of T. atroroseus LWT-1 through various chromatographic methods and identified as talaroconvolutins A (1) and B (2), and talarofuranone (3). Compounds 1 and 3 exhibited cytotoxic activity against MCF7, Huh-7, and H446 lines with IC50 values ranging from 0.68 ± 0.09 to 4.19 ± 0.71 μM. In contrast, compound 1 was non-toxic to BEAS-2B cells and significantly promoted their proliferation. To optimize pigments yield, we conducted a series of single-factor and orthogonal experiments to determine the optimal fermentation conditions. The optimal conditions were determined to be: SDA culture medium, 32°C incubation temperature, 170 rpm shaking speed, 60 mL working volume in a 250 mL flask, and a culture duration of 120 h.
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